Her martyrdom is considered to have occurred either during the persecution of Decius, in 251, or under Maximian in 286.
Honored in many Martyrologies, Regina's feast is celebrated on 7 September or in the Archdiocese of Paderborn on 20 June. In the past, a procession was held in her honor in the town of Dijon. However, her relics were transferred to Flavigny Abbey in 827. The history of the translation of Regina was the subject of a 9th-century account.
There are many places in France named Sainte-Reine after her.